QC Service Requests – RNA and Formulation Core

Capillary Electrophoresis RNA Analysis

Please note that we have recently updated our minimum requested sample requirements to 3.75 µg in 75 µL (50 ng/µL) to allow for sample re-testing.

Partner Information

Partner Type *UBC InternalExternal Academic/GovernmentIndustry

Name *

Email Address *

Request Details

Expected Sample Delivery Date *

Turnaround Time *1-4 Business Days1-20 Business Days

Quantity *

Price

Special Requests

Samples will be:

Purified and salt-free. *I agree.

What is your expected size? *We will not add our 15 nt oligo migration marker if there is a possibility for overlap. For extra-long RNA, we run an extended protocol.My RNA is an oligo/small (< 100 nt)My RNA is average (100 – 6000 nt)My RNA is large (> 6000 nt)Samples are various sizes (describe)

Prepared at 3.75 µg in 75 µL (50 ng/µL). *We require 25 µL per run. Will re-run free of charge if analysis fails – we can try to adjust for concentration or other sample issues. We will not re-run samples if there is not sufficient volume.I agree.

Clearly labelled in the following format: CE{submission#}.{sample number#} (CE22.1; CE22.2; CE22.3, etc). *Submission number will be emailed to you immediately upon submission.I agree.

dsRNA Slot Blot (dsRNA Content) Assay

Partner Information

Partner Type *UBC InternalExternal Academic/GovernmentIndustry

Name *

Email Address *

Request Details

Expected Sample Delivery Date *

Assay Type *High accuracy uses four 2-fold dilutions to increased accuracy and reproducibility, which is highly recommended when dsRNA content is unknown. High throughput assay is recommended for comparing relative dsRNA content between samples within a study.High Accuracy – 8 Sample MaxHigh Throughput – 32 Samples Max

Number of assays (not # of samples) *

Price

Sample Type *mRNAsaRNAcircRNAOther

Estimated dsRNA Content (if known)

Modified nucleotides or other modifications?

Samples will be:

Purified and salt-free. *I agree.

Prepared at 500 ng/µL, with a minimum volume of 6 µL. *I agree.

Clearly labelled by submission and sample number in format SB{SUBMISSION#}.{SAMPLE#} Example: SB22.1; SB22.2; SB22.3, etc. *Submission number will be emailed to you immediately upon submission.I agree.