RNA QC and Purification

While sequence and integrity are critical for the success of an RNA product, purity from endotoxin, double stranded RNA (dsRNA), DNA, and proteins are important factors for in vivo studies. As endotoxin and dsRNA are major immunostimulatory molecules, their role can range from beneficial to detrimental: from completely silencing translation to acting as an adjuvant for vaccines. Research groups embarking on drug development must be aware of the levels of such contaminants to ensure they are best prepared for success and effectively reproduce their success.

Ultra-high resolution RNA capillary electrophoresis analysis is available as a service. Details for internal UBC partners can be found here. External partners please inquire for more information and pricing here.

**World-class RNA functionality.** In vivo comparison of RNA produced by a local academic lab, a global leader, and the RNA core. No significant difference was observed for RNA produced by the RNA core and the global RNA leader. Transcript sequences were not controlled.

**Capillary electrophoresis provides superior resolution.** Resolution from gel electrophoresis (left) is insufficient to resolve two RNA species in solution. Capillary electrophoresis (right) both resolves and quantifies the mass % of each species in the sample.

QC Packages

QC	Standard	Deluxe
Visual Inspection	Included	Included
RNA Integrity & Length	Gel Electrophoresis	Capillary Electrophoresis
RNA Quantification	Photometric	Fluorometric
Salt Residue	Photometric	Photometric
Endotoxin Limit	1.0 EU/mL	0.1 EU/mL
Protein Residue	X	Fluorometric
DNA Template Quantification In development	X	qPCR
Capping Analysis In development	X	TBD
dsRNA Analysis In development	X	Dot Blot/ELISA
Bioburden In development	X	Growth @ 24hr

Deluxe Purification Packages In Development